PoplarV1, Main, Exploration, bibRecord, 001A78

Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.

Identifieur interne : 001A78 ( Main/Exploration ); précédent : 001A77; suivant : 001A79

Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.

Auteurs : Prashant S. Swamy [États-Unis] ; Hao Hu [États-Unis] ; Sivakumar Pattathil [États-Unis] ; Victoria J. Maloney [Canada] ; Hui Xiao [États-Unis] ; Liang-Jiao Xue [États-Unis] ; Jeng-Der Chung [Taïwan] ; Virgil E. Johnson [États-Unis] ; Yingying Zhu [États-Unis] ; Gary F. Peter [États-Unis] ; Michael G. Hahn [États-Unis] ; Shawn D. Mansfield [Canada] ; Scott A. Harding [États-Unis] ; Chung-Jui Tsai [États-Unis]

Source :

Journal of experimental botany [ 1460-2431 ] ; 2015.

RBID : pubmed:26246616

Descripteurs français

KwdFr :
- Maturation post-traductionnelle des protéines (MeSH), Paroi cellulaire (physiologie), Populus (génétique), Populus (physiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Stomates de plante (physiologie), Tubuline (génétique), Tubuline (métabolisme).
MESH :
- génétique : Populus, Protéines végétales, Tubuline.
- métabolisme : Protéines végétales, Tubuline.
- physiologie : Paroi cellulaire, Populus, Stomates de plante.
- Maturation post-traductionnelle des protéines, Régulation de l'expression des gènes végétaux.

English descriptors

KwdEn :
- Cell Wall (physiology), Gene Expression Regulation, Plant (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Stomata (physiology), Populus (genetics), Populus (physiology), Protein Processing, Post-Translational (MeSH), Tubulin (genetics), Tubulin (metabolism).
MESH :
- chemical , genetics : Plant Proteins, Tubulin.
- chemical , metabolism : Plant Proteins, Tubulin.
- genetics : Populus.
- physiology : Cell Wall, Plant Stomata, Populus.
- Gene Expression Regulation, Plant, Protein Processing, Post-Translational.

Abstract

Cortical microtubules are integral to plant morphogenesis, cell wall synthesis, and stomatal behaviour, presumably by governing cellulose microfibril orientation. Genetic manipulation of tubulins often leads to abnormal plant development, making it difficult to probe additional roles of cortical microtubules in cell wall biogenesis. Here, it is shown that expressing post-translational C-terminal modification mimics of α-tubulin altered cell wall characteristics and guard cell dynamics in transgenic Populus tremula x alba that otherwise appear normal. 35S promoter-driven transgene expression was high in leaves but unusually low in xylem, suggesting high levels of tubulin transgene expression were not tolerated in wood-forming tissues during regeneration of transformants. Cellulose, hemicellulose, and lignin contents were unaffected in transgenic wood, but expression of cell wall-modifying enzymes, and extractability of lignin-bound pectin and xylan polysaccharides were increased in developing xylem. The results suggest that pectin and xylan polysaccharides deposited early during cell wall biogenesis are more sensitive to subtle tubulin perturbation than cellulose and matrix polysaccharides deposited later. Tubulin perturbation also affected guard cell behaviour, delaying drought-induced stomatal closure as well as light-induced stomatal opening in leaves. Pectins have been shown to confer cell wall flexibility critical for reversible stomatal movement, and results presented here are consistent with microtubule involvement in this process. Taken together, the data show the value of growth-compatible tubulin perturbations for discerning microtubule functions, and add to the growing body of evidence for microtubule involvement in non-cellulosic polysaccharide assembly during cell wall biogenesis.

DOI: 10.1093/jxb/erv383
PubMed: 26246616
PubMed Central: PMC4588895

Affiliations:

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Le document en format XML

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<author><name sortKey="Xue, Liang Jiao" sort="Xue, Liang Jiao" uniqKey="Xue L" first="Liang-Jiao" last="Xue">Liang-Jiao Xue</name>
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<author><name sortKey="Johnson, Virgil E" sort="Johnson, Virgil E" uniqKey="Johnson V" first="Virgil E" last="Johnson">Virgil E. Johnson</name>
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<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Zhu, Yingying" sort="Zhu, Yingying" uniqKey="Zhu Y" first="Yingying" last="Zhu">Yingying Zhu</name>
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<country xml:lang="fr">États-Unis</country>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (physiology)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Stomata (physiology)</term>
<term>Populus (genetics)</term>
<term>Populus (physiology)</term>
<term>Protein Processing, Post-Translational (MeSH)</term>
<term>Tubulin (genetics)</term>
<term>Tubulin (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Maturation post-traductionnelle des protéines (MeSH)</term>
<term>Paroi cellulaire (physiologie)</term>
<term>Populus (génétique)</term>
<term>Populus (physiologie)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Stomates de plante (physiologie)</term>
<term>Tubuline (génétique)</term>
<term>Tubuline (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
<term>Tubulin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term>
<term>Tubulin</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term>
<term>Protéines végétales</term>
<term>Tubuline</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term>
<term>Tubuline</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Paroi cellulaire</term>
<term>Populus</term>
<term>Stomates de plante</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Wall</term>
<term>Plant Stomata</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Protein Processing, Post-Translational</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Maturation post-traductionnelle des protéines</term>
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<front><div type="abstract" xml:lang="en">Cortical microtubules are integral to plant morphogenesis, cell wall synthesis, and stomatal behaviour, presumably by governing cellulose microfibril orientation. Genetic manipulation of tubulins often leads to abnormal plant development, making it difficult to probe additional roles of cortical microtubules in cell wall biogenesis. Here, it is shown that expressing post-translational C-terminal modification mimics of α-tubulin altered cell wall characteristics and guard cell dynamics in transgenic Populus tremula x alba that otherwise appear normal. 35S promoter-driven transgene expression was high in leaves but unusually low in xylem, suggesting high levels of tubulin transgene expression were not tolerated in wood-forming tissues during regeneration of transformants. Cellulose, hemicellulose, and lignin contents were unaffected in transgenic wood, but expression of cell wall-modifying enzymes, and extractability of lignin-bound pectin and xylan polysaccharides were increased in developing xylem. The results suggest that pectin and xylan polysaccharides deposited early during cell wall biogenesis are more sensitive to subtle tubulin perturbation than cellulose and matrix polysaccharides deposited later. Tubulin perturbation also affected guard cell behaviour, delaying drought-induced stomatal closure as well as light-induced stomatal opening in leaves. Pectins have been shown to confer cell wall flexibility critical for reversible stomatal movement, and results presented here are consistent with microtubule involvement in this process. Taken together, the data show the value of growth-compatible tubulin perturbations for discerning microtubule functions, and add to the growing body of evidence for microtubule involvement in non-cellulosic polysaccharide assembly during cell wall biogenesis. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26246616</PMID>
<DateCompleted><Year>2016</Year>
<Month>07</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>66</Volume>
<Issue>20</Issue>
<PubDate><Year>2015</Year>
<Month>Oct</Month>
</PubDate>
</JournalIssue>
<Title>Journal of experimental botany</Title>
<ISOAbbreviation>J Exp Bot</ISOAbbreviation>
</Journal>
<ArticleTitle>Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.</ArticleTitle>
<Pagination><MedlinePgn>6507-18</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erv383</ELocationID>
<Abstract><AbstractText>Cortical microtubules are integral to plant morphogenesis, cell wall synthesis, and stomatal behaviour, presumably by governing cellulose microfibril orientation. Genetic manipulation of tubulins often leads to abnormal plant development, making it difficult to probe additional roles of cortical microtubules in cell wall biogenesis. Here, it is shown that expressing post-translational C-terminal modification mimics of α-tubulin altered cell wall characteristics and guard cell dynamics in transgenic Populus tremula x alba that otherwise appear normal. 35S promoter-driven transgene expression was high in leaves but unusually low in xylem, suggesting high levels of tubulin transgene expression were not tolerated in wood-forming tissues during regeneration of transformants. Cellulose, hemicellulose, and lignin contents were unaffected in transgenic wood, but expression of cell wall-modifying enzymes, and extractability of lignin-bound pectin and xylan polysaccharides were increased in developing xylem. The results suggest that pectin and xylan polysaccharides deposited early during cell wall biogenesis are more sensitive to subtle tubulin perturbation than cellulose and matrix polysaccharides deposited later. Tubulin perturbation also affected guard cell behaviour, delaying drought-induced stomatal closure as well as light-induced stomatal opening in leaves. Pectins have been shown to confer cell wall flexibility critical for reversible stomatal movement, and results presented here are consistent with microtubule involvement in this process. Taken together, the data show the value of growth-compatible tubulin perturbations for discerning microtubule functions, and add to the growing body of evidence for microtubule involvement in non-cellulosic polysaccharide assembly during cell wall biogenesis. </AbstractText>
<CopyrightInformation>© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Swamy</LastName>
<ForeName>Prashant S</ForeName>
<Initials>PS</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hu</LastName>
<ForeName>Hao</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA Department of Genetics, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pattathil</LastName>
<ForeName>Sivakumar</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Maloney</LastName>
<ForeName>Victoria J</ForeName>
<Initials>VJ</Initials>
<AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xiao</LastName>
<ForeName>Hui</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Laboratory for Macromolecular Analysis and Proteomics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xue</LastName>
<ForeName>Liang-Jiao</ForeName>
<Initials>LJ</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA Department of Genetics, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chung</LastName>
<ForeName>Jeng-Der</ForeName>
<Initials>JD</Initials>
<AffiliationInfo><Affiliation>Division of Silviculture, Taiwan Forestry Research Institute, Taipei 10066, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Johnson</LastName>
<ForeName>Virgil E</ForeName>
<Initials>VE</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA Department of Genetics, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Yingying</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA Department of Genetics, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Peter</LastName>
<ForeName>Gary F</ForeName>
<Initials>GF</Initials>
<AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, Gainesville, FL 32611, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hahn</LastName>
<ForeName>Michael G</ForeName>
<Initials>MG</Initials>
<AffiliationInfo><Affiliation>Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mansfield</LastName>
<ForeName>Shawn D</ForeName>
<Initials>SD</Initials>
<AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Harding</LastName>
<ForeName>Scott A</ForeName>
<Initials>SA</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA Department of Genetics, University of Georgia, Athens, GA 30602, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tsai</LastName>
<ForeName>Chung-Jui</ForeName>
<Initials>CJ</Initials>
<AffiliationInfo><Affiliation>School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA Department of Genetics, University of Georgia, Athens, GA 30602, USA cjtsai@uga.edu.</Affiliation>
</AffiliationInfo>
</Author>
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<Language>eng</Language>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
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<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>08</Month>
<Day>05</Day>
</ArticleDate>
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<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Exp Bot</MedlineTA>
<NlmUniqueID>9882906</NlmUniqueID>
<ISSNLinking>0022-0957</ISSNLinking>
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<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
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<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName>
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<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D054046" MajorTopicYN="N">Plant Stomata</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
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<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
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<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
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<MeshHeading><DescriptorName UI="D011499" MajorTopicYN="Y">Protein Processing, Post-Translational</DescriptorName>
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<Keyword MajorTopicYN="N">detyrosination</Keyword>
<Keyword MajorTopicYN="N">guard cell</Keyword>
<Keyword MajorTopicYN="N">microtubule</Keyword>
<Keyword MajorTopicYN="N">polysaccharide</Keyword>
<Keyword MajorTopicYN="N">tubulin post translational modification.</Keyword>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Plant Cell Physiol. 2004 Feb;45(2):211-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14988491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Diagn. 2003 May;5(2):73-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12707371</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1990 Oct;10(10):5295-304</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2204812</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2009 Apr;21(4):1141-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19376932</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Sci. 2002 Apr 1;115(Pt 7):1345-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11896182</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2012 Apr;53(4):699-708</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22383625</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2006;344:143-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17033059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1989 Mar;9(3):1049-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2657385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2006;57:859-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16669785</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2002 May;53(371):1067-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11971918</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2007 Dec;6(12):2058-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17848588</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1987 Oct 23;51(2):283-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2444342</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2013 Nov;76(3):357-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23889038</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(8):e44467</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22952983</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 1993 Feb;120(3):725-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8093886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2012 Feb 24;287(9):6503-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22170066</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2005 May;221(2):255-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15578215</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2011 Dec;12(12):773-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22086369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2012 Jan;24(1):50-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22247250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 1999 Jul;17(7):712-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10404167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2007 May 15;104(20):8544-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17488810</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2010 Jan;9(1):359-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19886702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 1997;201(3):349-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9129339</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 1963 Oct 1;19(1):239-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19866635</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2003;54:519-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14503002</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1994;230:1-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8139491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2013 Dec;163(4):1804-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24134884</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Jun;153(2):514-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20363856</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 1996 Dec;135(6 Pt 1):1525-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8978820</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2008 Jan;6(1):62-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17908207</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2000 Jul;18(7):784-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10888850</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1991 Oct 29;30(43):10523-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1931974</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Jun 9;312(5779):1491-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16627697</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2014 Jul 28;5:357</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25120548</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics Clin Appl. 2012 Oct;6(9-10):502-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22996942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2015 Jul;8(7):1011-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25600279</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2014 Jan 22;7(1):11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24450583</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2004 Jan;218(3):460-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14534789</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1984 Oct;38(3):779-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6386177</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechniques. 2003 Feb;34(2):374-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12613259</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Nov;25(11):4324-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24280390</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2015 Mar;167(3):780-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25646318</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Neurosci. 2009 May;12(5):559-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19377471</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2012;908:61-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22843389</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2013 Jul;75(2):219-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23551562</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2010 Apr 27;20(8):744-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20399097</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 1998 Jan;39(1):80-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9517004</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2008 May;227(6):1363-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18309515</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1987 Dec;84(24):9040-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3321065</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Sep;25(9):3329-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24045021</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2011 Jan 15;27(2):225-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21098430</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2012 May;5(3):601-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22402260</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2011 Nov;157(3):1452-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21908685</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Neurosci. 2010 Aug;33(8):362-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20541813</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2014 Feb;65(2):465-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24285826</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 1998 Apr 17;245(2):349-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9571153</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Cell Biol. 2014 Apr;16(4):335-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24633327</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2012 Mar;7(3):562-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22383036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2008 Jun;13(6):303-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18467155</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2003 Feb;33(4):677-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12609041</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Cell Biol. 2013;113:339-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23317910</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Oct;154(2):483-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20921169</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2011 Mar;4(2):331-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21300756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2007 Nov;145(3):961-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17885081</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2014 Aug;65(15):4191-200</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24803501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11783-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">13130074</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2010 Nov;64(4):657-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21070417</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Cell Biol. 2009 Jul;11(7):797-806</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19525940</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2013 Feb;197(3):777-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23278123</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 2009 Jun 29;185(7):1159-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19564401</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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   |texte=   Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.
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Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.

Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.

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